SH JAMSHIDI; B FARAHMAND; A AKBARZADEH; B ,LAMEH RAD
Volume 12, Issue 4 , January and February 2006, , Pages 6-13
Abstract
Background and Purpose: Although it is now certain that insulin injection allows us to control the complications resulting from blood sugar imbalances, its accurate control by insulin injection is difficult. Therefore as a logical solution, transplantation of islet cells is globally considered as a treatment ...
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Background and Purpose: Although it is now certain that insulin injection allows us to control the complications resulting from blood sugar imbalances, its accurate control by insulin injection is difficult. Therefore as a logical solution, transplantation of islet cells is globally considered as a treatment and the present study is conducted to verify it.
Methods and Materials: After materials preparation, donor tissue was obtained from 6 male wistar rate cage 75-90 days (weight 250-300 gram). Transplantation was done on diabetic rate induced 2-4 weeks earlier by 60 mg/kg streptozotocin intravenous injection.
Results: After islet cells transplantation, blood glucose was reduced to normal (145±5 mg/dl) and insulin and C-peptide increased (1.9±0.1 MIU/L and 0.053±0.001 mg/ml respectively). Clinical symptoms of dibetes induction were relieved after transplantation.
Conclusion: The technique of islet cells transplantation in the form of encapsulation with the absence of immunological inhibitors to support the graft against the recipient's immunity system is an innovative method to treat type I diabetes.
SH JAMSHIDI; A FARHANGI; B LAMERAD; A AKBARZADEH; S BAHREMAND
Volume 12, Issue 2 , July and August 2005, , Pages 6-13
Abstract
Background and Purpose: It is thought that transplantation of islet cells would cure diabetic patients in world. Islet cells transplantation in people suffering from diabetes is of technical interest. A standardized procedure was developed for the preparation of rat islet cell grafts for purification ...
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Background and Purpose: It is thought that transplantation of islet cells would cure diabetic patients in world. Islet cells transplantation in people suffering from diabetes is of technical interest. A standardized procedure was developed for the preparation of rat islet cell grafts for purification of islet cells.
Materials and methods: In this process, after collagens digestion of pancreases, islets were isolated and dissociated, then with enzymatic procedure by DNase and trypsin, the islet cells changed in to single cells and these cells were assayed by flow cytometery.
Results: Flow cytometery of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles were lost during digestion.
Conclusion: Purified endocrine islet cell grafts were prepared by pure beta-cells, with or without endocrine non-beta cells. The purified aggregates were devoid of non-endocrine cells and damaged cells.